ABSTRACT
With the prevalence of COVID-19, wearing medical surgical masks has become a requisite measure to protect against the invasion of the virus. Therefore, a huge amount of discarded medical surgical masks will be produced, which will become a potential hazard to pollute the environment and endanger the health of organisms without our awareness. Herein, a green and cost-effective way for the reasonable disposal of waste masks becomes necessary. In this work, we realized the transformation from waste medical surgical masks into high-quality carbon-nickel composite nanowires, which not only benefit the protection of the environment and ecosystem but also contribute to the realization of economic value. The obtained composite carbon-based materials demonstrate 70 S m-1 conductivity, 5.2 nm average pore diameters, 234 m2 g-1 surface areas, and proper graphitization degree. As an anode material for lithium-ion batteries, the prepared carbon composite materials demonstrate a specific capacity of 420 mA h g-1 after 800 cycles at a current density of 0.2 A g-1. It also displays good rate performance and decent cycling stability. Therefore, this study provides an approach to converting the discarded medical surgical masks into high-quality carbon nanowire anode materials to turn waste into treasure.
ABSTRACT
Aim Human TMPRSS2 is a transmembrane serine protease.In this paper, the structure and function of the protein were systematically analyzed by bioinformatics, the codon was optimized and the pro- karvotie expression vector was constructed to explore the molecular mechanism of SARS-CoV-2 infecting host cells.Methods The recombinant expression vector pET-22b-TMPRSS2 was generated by molecular cloning technology.The homology, functional sites, subcellular localization, three-dimensional structure and evolutionary characteristics of TMPRSS2 protein were systematically analyzed by using analytical tools such as Protparam, NetPhos3.1, Blast, Clustal X2 and MEGA7.0.Results The prokarvotic expression plas- mid was constructed correctly;TMPRSS2 belongs to medium molecular weight protein, which is composed of 492 amino acid residues.The theoretical isoelectric point is 8.12, the molecular extinction coefficient is 118 145 L * mol~1 * cm"1 , and the half-life is 30 h;TMPRSS2 has 15 potential glycosylation sites and 49 possible phosphorylation sites.It is a transmembrane hydrophilie protein without signal sequenee.In addition, the protein has 13 potential B-cell epitopes and 7 T-eell epitopes.Seeondarv structure analysis showed that random coil accounted for the highest proportion of TMPRSS2 protein ( 0.453 3) , followed by extended strand (0.252 0).Sequence comparison and evolutionary analysis showed that the highest sequence consistency and closest genetic relationship with human TMPRSS2 was Pan troglodytes, followed by gorilla.Conclusions Human-derived TMPRSS2 protein is ev- olutionarilv conserved and functionally important.Hie results of this study can help to reveal the structure and mechanism of action of TMPRSS2 protein, provide ideas for the diagnosis and treatment of COYID-19, and accelerate the research and development process of new drugs targeting TMPRSS2 protein. Copyright © 2022 Publication Centre of Anhui Medical University. All rights reserved.
ABSTRACT
The movement restrictions of coronavirus had a significant impact on consumer behavior. The rise of cross-border e-commerce has also increased the risks in logistics activities. This work identified the critical threat of cross-border logistics. Additionally, artificial intelligence over Internet of Things (AIoT) technology was found to reduce logistics risk in this work beneficially. © 2022 IEEE.